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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDGFRP3 Polyclonal antibody (12380-1-AP)

    WB analysis using 12380-1-AP (same clone as 12380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IP experiment of mouse testis using 12380-1-AP (same clone as 12380-1-PBS)

    IP result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IHC staining of human prostate cancer using 12380-1-AP (same clone as 12380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HDGFRP3 Polyclonal antibody (12380-1-AP)

    IF Staining of MCF-7 using 12380-1-AP (same clone as 12380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HDGFRP3 antibody (12380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12380-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HDGFRP3 Polyclonal antibody, PBS Only

HDGFRP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HDGF-2, HDGF2, HDGF 2, CGI-142, CGI142

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12380-1-PBS targets HDGFRP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3046

Product name: Recombinant human HDGFRP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC015483

Sequence: MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT

相同性解析による交差性が予測される生物種
Full Name hepatoma-derived growth factor, related protein 3
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015483
Gene Symbol HDGFRP3
Gene ID (NCBI) 50810
RRIDAB_2117222
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3E1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is a 30 kDa protein for phosphor modification.

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