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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HDGF Monoclonal antibody (60064-2-Ig)

    WB analysis of HeLa using 60064-2-Ig

    WB result of HDGF antibody (60064-2-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDGF transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using HDGF Monoclonal antibody (60064-2-Ig)

    WB analysis using 60064-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HDGF Monoclonal antibody (60064-2-Ig)

    WB analysis using 60064-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • KD/KO Validated

HDGF Monoclonal antibody

HDGF Monoclonal Antibody for WB, ELISA
Cat No. 60064-2-Ig
Clone No.1A10D5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, Caki-1 cells, SCaBER cells, MG-63 cells, MCF-10A cells, MCF-7 cells, MOLT-4 cells, Karpas-299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60064-2-Ig targets HDGF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1893

Product name: Recombinant human HDGF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC018991

Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

相同性解析による交差性が予測される生物種
Full Name hepatoma-derived growth factor (high-mobility group protein 1-like)
Calculated molecular weight 26 kDa
Observed molecular weight40 kDa
GenBank accession numberBC018991
Gene Symbol HDGF
Gene ID (NCBI) 3068
RRIDAB_3743210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)

Protocols

Product Specific Protocols
WB protocol for HDGF antibody 60064-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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