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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDDC2 Polyclonal antibody

HDDC2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16632-1-AP

Synonyms

C6orf74, CGI 130, HD domain containing 2, HD domain containing protein 2, HDDC2, NS5ATP2

Validation Data Gallery

Applications:
  • WB analysis using 16632-1-AP

    WB analysis using 16632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16632-1-AP (HDDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IHC staining of human brain using 16632-1-AP

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HDDC2 antibody included
  • IF Staining of PC-3 using 16632-1-AP

    IF Staining of PC-3 using 16632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16632-1-AP (HDDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy HDDC2 Ready-To-Use IHC Kit. HDDC2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16632-1-AP targets HDDC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDDC2 fusion protein Ag9971 相同性解析による交差性が予測される生物種
Full Name HD domain containing 2
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC003357
Gene symbol HDDC2
Gene ID (NCBI) 51020
RRIDAB_10597248
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

Protocols

Product Specific Protocols
WB protocol for HDDC2 antibody 16632-1-APDownload protocol
IHC protocol for HDDC2 antibody 16632-1-APDownload protocol
IF protocol for HDDC2 antibody 16632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols