Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10255-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 10255-1-AP
WB result of HDAC3 antibody (10255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3 transfected U-251 cells.
WB analysis using 10255-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 using 10255-1-AP
WB result of HDAC3 antibody (10255-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and HDAC3 knockout HAP1 cells.
WB analysis using 10255-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10255-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10255-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10255-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10255-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10255-1-AP
IP result of anti-HDAC3 (IP:10255-1-AP, 3ug; Detection:10255-1-AP 1:300) with A431 cells lysate 1200ug.
IHC staining of human ovary tumor using 10255-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10255-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of K-562 using 10255-1-AP
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HDAC3 antibody (10255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

HDAC3 Polyclonal antibody

HDAC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10255-1-AP

Publications(68)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Synonyms

EC:3.5.1.-, EC:3.5.1.98, HDAC 3, Histone deacetylase 3, Protein deacetylase HDAC3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (68)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, HAP1 cells, HL-60 cells, U-251 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, bEnd.3 cells, NIH/3T3 cells, C6 cells, mouse testis tissue, rat testis tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 57 publications below
IHC	See 9 publications below
IF	See 7 publications below
IP	See 4 publications below
CoIP	See 3 publications below
ChIP	See 2 publications below

Product Information

10255-1-AP targets HDAC3 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0396 Product name: Recombinant human HDAC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 187-395 aa of BC000614 Sequence: VMTVSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKHLFQPVINQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVKSFNIPLLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADA 相同性解析による交差性が予測される生物種
Full Name	histone deacetylase 3
Calculated molecular weight	49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC000614
Gene Symbol	HDAC3
Gene ID (NCBI)	8841
RRID	AB_2279733
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15379
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.

Protocols

Product Specific Protocols
WB protocol for HDAC3 antibody 10255-1-AP	Download protocol
IHC protocol for HDAC3 antibody 10255-1-AP	Download protocol
IF protocol for HDAC3 antibody 10255-1-AP	Download protocol
IP protocol for HDAC3 antibody 10255-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. Authors - Xin Sun View Article
mouse	WB	J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. Authors - Shanshan Zhong View Article
human	WB	Mol Ther Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis Authors - Chao Li View Article
mouse	WB	J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. Authors - Zhe Chen View Article
mouse	WB	J Hazard Mater Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits Authors - Mengmeng Wang View Article
rat	WB	Cell Rep Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex. Authors - Tian Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10255-1-AP

WB analysis of U-251 using 10255-1-AP

WB analysis using 10255-1-AP

WB analysis of HAP1 using 10255-1-AP

WB analysis using 10255-1-AP

WB analysis of HL-60 using 10255-1-AP

WB analysis of HeLa using 10255-1-AP

WB analysis of A431 using 10255-1-AP

WB analysis of HL-60 using 10255-1-AP

IP experiment of A431 using 10255-1-AP

IHC staining of human ovary tumor using 10255-1-AP

IHC staining of human ovary tumor using 10255-1-AP

IF Staining of K-562 using 10255-1-AP

HDAC3 Polyclonal antibody

HDAC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis

Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.