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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HDAC10 Recombinant antibody (83788-4-RR)

    WB analysis of Jurkat using 83788-4-RR (same clone as 83788-4-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 83788-4-RR (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83788-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83788-4-RR

    Affinity and Kinetic Characterization of 83788-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83788-4-RR against Human HDAC10 were performed. The affinity constant is 0.476 nM.

HDAC10 Recombinant monoclonal antibody, PBS Only

HDAC10 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83788-4-PBS
Clone No.240877E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83788-4-PBS targets HDAC10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18851

Product name: Recombinant human HDAC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 320-669 aa of BC125083

Sequence: TLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA

相同性解析による交差性が予測される生物種
Full Name histone deacetylase 10
Calculated molecular weight 669 aa, 71 kDa
GenBank accession numberBC125083
Gene Symbol HDAC10
Gene ID (NCBI) 83933
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ969S8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.