Validation Data Gallery
Tested Applications
| Positive WB detected in | C6 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32859-1-AP targets HCRTR2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38128 Product name: Recombinant human HCRTR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 380-444 aa of NM_001526 Sequence: SCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW 相同性解析による交差性が予測される生物種 |
| Full Name | hypocretin (orexin) receptor 2 |
| Calculated molecular weight | 440aa, 51 kDa |
| Observed molecular weight | 70-73 kDa |
| GenBank accession number | NM_001526 |
| Gene Symbol | HCRTR2 |
| Gene ID (NCBI) | 3062 |
| RRID | AB_3742766 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O43614 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HCRTR2 encodes hypocretin receptor 2, a G protein-coupled receptor that binds the neuropeptides orexin-A and orexin-B with high affinity. Through this binding, it activates G(q)/11 and G(i)/o signaling to modulate cytoplasmic Ca2+ levels and neuronal excitability, placing it at the center of sleep-wake cycle regulation and central glucose homeostasis. Protein expression is high in the brain, consistent with its role in neural control of arousal and metabolism.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HCRTR2 antibody 32859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |