Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86879-3-RR targets HBZ in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11061 Product name: Recombinant human HBZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC027892 Sequence: MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAARFPADFTAEAHAAWDKFLSVVSSVLTEKYR 相同性解析による交差性が予測される生物種 |
| Full Name | hemoglobin, zeta |
| Calculated molecular weight | 142 aa, 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC027892 |
| Gene Symbol | HBZ |
| Gene ID (NCBI) | 3050 |
| RRID | AB_3745181 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02008 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HBZ, also named as Zeta-globin and HBAZ, belongs to the globin family. HBZ is an alpha-type chain of mammalian embryonic hemoglobin, synthesized primarily in the yolk sac. HBZ was first identified as an antisense viral protein that associated with cAMP-response element binding protein-2 (CREB-2) in HTLV-1-infected cells. HBZ is the only viral gene that is expressed in all ATL cases (PMID: 22787458). ShRNA repression of HBZ expression in established HTLV-1-transformed cell lines and newly immortalized T-lymphocytes also significantly suppressed T-lymphocyte proliferation (PMID: 19650892, 16917009).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HBZ antibody 86879-3-RR | Download protocol |
| WB protocol for HBZ antibody 86879-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |