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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis of HepG2 using 68146-1-Ig (same clone as 68146-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis of HEK-293 using 68146-1-Ig (same clone as 68146-1-PBS)

    WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HBS1L Monoclonal antibody (68146-1-Ig)

    IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HBS1L Monoclonal antibody (68146-1-Ig)

    IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

HBS1L Monoclonal antibody, PBS Only

HBS1L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68146-1-PBS
Clone No.1A10C4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68146-1-PBS targets HBS1L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0388

Product name: Recombinant human HBS1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-262 aa of BC001465

Sequence: SRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPGVSSEENGHSFHTPQKGPPIEDAIASSDVLETASKSANPPHTIQASEEQSSTPAPVKKSGKLRQQIDVKAELEKRQGGKQLLN

相同性解析による交差性が予測される生物種
Full Name HBS1-like (S. cerevisiae)
Calculated molecular weight 75 kDa
Observed molecular weight75 kDa
GenBank accession numberBC001465
Gene Symbol HBS1L
Gene ID (NCBI) 10767
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y450
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

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