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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using HBQ1 Recombinant monoclonal antibody (83576-4-RR)

    WB analysis of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using HBQ1 Recombinant monoclonal antibody (83576-4-RR)

    IF Staining of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBQ1 antibody (83576-4-RR, Clone: 240527D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83576-4-PBS

    Affinity and Kinetic Characterization of 83576-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83576-4-PBS against Human HBQ1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83576-4-RR

    Affinity and Kinetic Characterization of 83576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

HBQ1 Recombinant monoclonal antibody, PBS Only

HBQ1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83576-4-PBS
Clone No.240527D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83576-4-PBS targets HBQ1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10105

Product name: Recombinant human HBQ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC056686

Sequence: MALSAEDRALVRALWKKLGSNVGVYTTEALERTFLAFPATKTYFSHLDLSPGSSQVRAHGQKVADALSLAVERLDDLPHALSALSHLHACQLRVDPASFQLLGHCLLVTLARHYPGDFSPALQASLDKFLSHVISALVSEYR

相同性解析による交差性が予測される生物種
Full Name hemoglobin, theta 1
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight10~16 kDa
GenBank accession numberBC056686
Gene Symbol HBQ1
Gene ID (NCBI) 3049
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP09105
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

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