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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBG1 Recombinant monoclonal antibody (86851-1-RR)

    WB analysis using 86851-1-RR (same clone as 86851-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86851-1-RR (HBG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86851-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using HBG1 Recombinant monoclonal antibody (86851-1-RR)

    IF Staining of K-562 using 86851-1-RR (same clone as 86851-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBG1 antibody (86851-1-RR, Clone: 251754H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86851-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86851-1-RR

    Affinity and Kinetic Characterization of 86851-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86851-1-RR against Human HBG1 were performed. The affinity constant is 0.941 nM.

HBG1 Recombinant monoclonal antibody, PBS Only

HBG1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86851-1-PBS
Clone No.251754H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Gamma 1 globin, Gamma-1-globin, Hb F Agamma, HBGA, HBGR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86851-1-PBS targets HBG1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22776

Product name: Recombinant human HBG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC010913

Sequence: MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDATKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIH

相同性解析による交差性が予測される生物種
Full Name hemoglobin, gamma A
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC010913
Gene Symbol HBG1
Gene ID (NCBI) 3047
RRIDAB_3745163
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP69891
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HBG1, also named as Hb F Agamma, HBGA, HBGR and HSGGL, belongs to the globin family. HBG1 makes up the fetal hemoglobin F, in combination with alpha chains. Some gamma variants can cause severe jaundice and cyanosis in premature and new born babies. The antibody is specific to HBG1 and HBG2. The antibody has no cross reaction to HBB and HBD. HBG1 protein migrates around 16 kDa. Heterodimer of 32 kDa may also be observed. This antibody could identify HBG1 and HBG2.

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