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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIM-1/HAVCR1 Polyclonal antibody

KIM-1/HAVCR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30948-1-AP

Synonyms

CD365, Havcr1, KIM 1, KIM1, TIM-1, TIMD-1

Validation Data Gallery

Applications:
  • WB analysis using 30948-1-AP

    WB analysis using 30948-1-AP

    Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 30948-1-AP

    IHC staining of rat kidney using 30948-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 30948-1-AP

    IHC staining of rat kidney using 30948-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30948-1-AP targets KIM-1/HAVCR1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name hepatitis A virus cellular receptor 1
Calculated molecular weight34 kDa
Observed molecular weight72 kDa
GenBank accession numberNM_173149.2
Gene symbol Havcr1
Gene ID (NCBI) 286934
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). KIM-1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

Protocols

Product Specific Protocols
WB protocol for KIM-1/HAVCR1 antibody 30948-1-APDownload protocol
IHC protocol for KIM-1/HAVCR1 antibody 30948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols