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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HAUS1 Polyclonal antibody (11094-2-AP)

    WB analysis using 11094-2-AP (same clone as 11094-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HAUS1 Polyclonal antibody (11094-2-AP)

    IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

    IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HAUS1 Polyclonal antibody (11094-2-AP)

    IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

HAUS1 Polyclonal antibody, PBS Only

HAUS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11094-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CCDC5, Coiled-coil domain-containing protein 5, Enhancer of invasion cluster, Enhancer of invasion-cluster, HAUS augmin-like complex subunit 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11094-2-PBS targets HAUS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1569

Product name: Recombinant human CCDC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-278 aa of BC014003

Sequence: MEPQEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAKYLQDLLMESVNFSPANLSSTGSRYLNALVDSAVALETKDTSLASFIPAVNDLTSDLFRTKSKSEEIKIELEKLEKNLTATLVLEKCLQEDVKKAELHLSTERAKVDNRRQNMDFLKAKSEEFRFGIKAAEEQLSARGMDASLSHQSLVALSEKLARLKQQTIPLKKKLESYLDLMPNPSLAQVKIEEAKRELDSIEAELTRRVDMMEL

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 5 (spindle associated)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC014003
Gene Symbol HAUS1
Gene ID (NCBI) 115106
RRIDAB_614986
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CS2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

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