Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, NIH/3T3 cells, PC-12 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00092 targets HAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | histone acetyltransferase 1 |
| Calculated molecular weight | 419 aa, 50 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC018682 |
| Gene Symbol | HAT1 |
| Gene ID (NCBI) | 8520 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | O14929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HAT1 (also known as KAT1) is located on human chromosome 2q31.1 and encodes histone acetyltransferase 1. This gene is widely expressed in various tissues, and the protein is predominantly localized nuclear in normal cells. Its primary function is to acetylate newly synthesized histones H4 (Lys5, Lys12) and H2A (Lys5), thereby promoting chromatin assembly and playing a key role in DNA replication, cell cycle progression, and DNA damage repair. This antibody can detect a band around 46 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HAT1 antibody RMX00092 | Download protocol |
| IHC protocol for HAT1 antibody RMX00092 | Download protocol |
| WB protocol for HAT1 antibody RMX00092 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |