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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAS3 Polyclonal antibody (15609-1-AP)

    WB analysis using 15609-1-AP (same clone as 15609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using HAS3 Polyclonal antibody (15609-1-AP)

    WB analysis of L02 using 15609-1-AP (same clone as 15609-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using HAS3 Polyclonal antibody (15609-1-AP)

    WB analysis of mouse liver using 15609-1-AP (same clone as 15609-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HAS3 Polyclonal antibody (15609-1-AP)

    IHC staining of human liver cancer using 15609-1-AP (same clone as 15609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HAS3 Polyclonal antibody (15609-1-AP)

    IHC staining of human liver cancer using 15609-1-AP (same clone as 15609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15609-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HAS3 Polyclonal antibody, PBS Only

HAS3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15609-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15609-1-PBS targets HAS3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7999

Product name: Recombinant human HAS3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-281 aa of BC021853

Sequence: MPVQLTTALRVVGTSLFALAVLGGILAAYVTGYQFIHTEKHYLSFGLYGAILGLHLLIQSLFAFLEHRRMRRAGQALKLPSPRRGSVALCIAAYQEDPDYLRKCLRSAQRISFPDLKVVMVVDGNRQEDAYMLDIFHEVLGGTEQAGFFVWRSNFHEAGEGETEASLQEGMDRVRDVVRASTFSCIMQKWGGKREVMYTAFKALGDSVDYIQVCDSDTVLDPACTIEMLRVLEEDPQVGGVGGDVQPPGKGMAVEDDQVQAAQVRATEAWSVHQRHVSREQ

相同性解析による交差性が予測される生物種
Full Name hyaluronan synthase 3
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC021853
Gene Symbol HAS3
Gene ID (NCBI) 3038
RRIDAB_2232886
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00219
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HAS3(hyaluronan synthase 3) belongs to the NodC/HAS family, which are widely expressed in human tissues and appear to function in embryogenesis, wound healing and other processes associated with cellular proliferation and migration(PMID:14566823). HAS3 is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. It may play a role in the progression of colon cancer and, as such, may provide novel targets for diagnostic and therapeutic interventions(PMID:14566823). It has 2 isoforms produced by alternative splicing and the protein has a glycosylation site.

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