Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HCT 116 cells, HT-29 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IF | See 1 publications below |
Product Information
83204-2-RR targets HAS2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34945 Product name: Recombinant human HAS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-170 aa of BC069353 Sequence: EHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSEDDLYMMDIFSEVMGRDKSATYIWKNNFHEKGPGETDESHKESSQH 相同性解析による交差性が予測される生物種 |
| Full Name | hyaluronan synthase 2 |
| Calculated molecular weight | 552 aa, 64 kDa |
| Observed molecular weight | 63-67 kDa |
| GenBank accession number | BC069353 |
| Gene Symbol | HAS2 |
| Gene ID (NCBI) | 3037 |
| RRID | AB_3670892 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92819 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HAS2 antibody 83204-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |