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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse cerebellum using 26831-1-AP (same clone as 26831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse cerebellum using 26831-1-AP (same clone as 26831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse placenta using 26831-1-AP (same clone as 26831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse placenta using 26831-1-AP (same clone as 26831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HAPLN1 Polyclonal antibody (26831-1-AP)

    IF Staining of U2OS using 26831-1-AP (same clone as 26831-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HAPLN1 antibody (26831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

HAPLN1 Polyclonal antibody, PBS Only

HAPLN1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 26831-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26831-1-PBS targets HAPLN1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25221

Product name: Recombinant human HAPLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 16-131 aa of BC057808

Sequence: DHLSDNYTLDHDRAIHIQAENGPHLLVEAEQAKVFSHRGGNVTLPCKFYRDPTAFGSGIHKIRIKWTKLTSDYLKEVDVFVSMGYHKKTYGGYQGRVFLKGGSDSDASLVITDLTL

相同性解析による交差性が予測される生物種
Full Name hyaluronan and proteoglycan link protein 1
Calculated molecular weight 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC057808
Gene Symbol HAPLN1
Gene ID (NCBI) 1404
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10915
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453).

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