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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using HAP1 Polyclonal antibody (25133-1-AP)

    WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using HAP1 Polyclonal antibody (25133-1-AP)

    WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HAP1 Polyclonal antibody (25133-1-AP)

    IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HAP1 Polyclonal antibody (25133-1-AP)

    IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using HAP1 Polyclonal antibody (25133-1-AP)

    IF Staining of SH-SY5Y using 25133-1-AP (same clone as 25133-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 25133-1-AP (HAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

HAP1 Polyclonal antibody, PBS Only

HAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25133-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25133-1-PBS targets HAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17705

Product name: Recombinant human HAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 331-671 aa of BC156728

Sequence: LREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEESVWVGSQLQDLREKYMDCGGMLIEMQEEVKTLRQQPPVSTGSATHYPYSVPLETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR

相同性解析による交差性が予測される生物種
Full Name huntingtin-associated protein 1
Calculated molecular weight 671 aa, 76 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC156728
Gene Symbol HAP1
Gene ID (NCBI) 9001
RRIDAB_2879915
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54257
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).

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