Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10882-1-AP targets HAND1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1324 Product name: Recombinant human HAND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC021190 Sequence: MNLVGSYAHHHHHHHPHPAHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPAAAAAATAYGPDARPGQSPGRLEALGGRLGRRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAQSGDPEAFKAELKKADGGRESKRKRELQQHEGFPPALGPVEKRIKGRTGWPQQVWALELNQ 相同性解析による交差性が予測される生物種 |
| Full Name | heart and neural crest derivatives expressed 1 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC021190 |
| Gene Symbol | HAND1 |
| Gene ID (NCBI) | 9421 |
| RRID | AB_3741815 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O96004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The HAND1 gene encodes a basic helix-loop-helix (bHLH) transcription factor essential for mammalian heart development (PMID:19586923). HAND1 is highly expressed in the heart and in cytotrophoblastic cell lines, and is required in the adult for sustained expression of cardiac-specific genes (PMID:9931445).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HAND1 antibody 10882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |