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SPINT1/HAI-1 Monoclonal antibody

SPINT1/HAI-1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67031-1-Ig
Clone No.2H2D8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ChIP, ELISA

HAI 1, HAI-1, SPINT1, 2H2D8, HAI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman placenta tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67031-1-Ig targets SPINT1/HAI-1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPINT1/HAI-1 fusion protein Ag26733 相同性解析による交差性が予測される生物種
Full Name serine peptidase inhibitor, Kunitz type 1
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC004140
Gene Symbol HAI-1
Gene ID (NCBI) 6692
RRIDAB_2882346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).

Protocols

Product Specific Protocols
WB protocol for SPINT1/HAI-1 antibody 67031-1-IgDownload protocol
IF protocol for SPINT1/HAI-1 antibody 67031-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,ChIP

J Cell Biol

Matriptase drives dissemination of ovarian cancer spheroids by a PAR-2/PI3K/Akt/MMP9 signaling axis

Authors - Nisha R Pawar
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