Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, PANC-1 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
17196-1-AP targets GLO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9998 Product name: Recombinant human HAGH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC000840 Sequence: MKVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxyacylglutathione hydrolase |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC000840 |
| Gene Symbol | HAGH |
| Gene ID (NCBI) | 3029 |
| RRID | AB_2878361 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16775 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLO2 (glyoxalase 2), also known as hydroxyacylglutathione hydrolase (HAGH), is a crucial component of the glyoxalase system that catalyzes the hydrolysis of S-D-lactoylglutathione to regenerate glutathione and release D-lactate, thereby completing methylglyoxal detoxification and protecting cells from glycation damage (PMID: 2198020).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLO2 antibody 17196-1-AP | Download protocol |
| IHC protocol for GLO2 antibody 17196-1-AP | Download protocol |
| WB protocol for GLO2 antibody 17196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Targeting Methylglyoxal Metabolism to Enhance Ferroptosis Sensitivity in Tumor Therapy | ||
Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. | ||
Oncol Rep Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cells |