Validation Data Gallery
Tested Applications
| Positive WB detected in | A-204 cells, DU 145 cells, HepG2 cells, PANC-1 cells, SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33317-1-AP targets HABP4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39922 Product name: Recombinant human HABP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-413 aa of NM_014282.3 Sequence: LEVEEETQVQEMTLDEWKNLQEQTRPKPEFNIRKPESTVPSKAVVIHKSKYRDDMVKDDYEDDSHVFRKPANDITSQLEINFGNLPRPGRGARGGTRGGRGRIRRAENYGPRAEVVMQDVAPNPDDPEDFPALS 相同性解析による交差性が予測される生物種 |
| Full Name | hyaluronan binding protein 4 |
| Calculated molecular weight | 46kDa，413aa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | NM_014282.3 |
| Gene Symbol | HABP4 |
| Gene ID (NCBI) | 22927 |
| RRID | AB_3742938 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5JVS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HABP4 (hyaluronic acid binding protein 4) is an intracellular regulatory protein, with a molecular weight of about 57 kDa (PMID: 33245729), which is rich in positively charged amino acids and can bind with negatively charged macromolecules such as RNA and hyaluronic acid. It has SUMO binding activity, participates in PML tissue, negatively regulates DNA binding activity, and positively regulates gene expression. In addition, HABP4 has a complex interaction network in cells, and has undergone a variety of post-translational modifications, including serine/threonine phosphorylation, arginine methylation and SUMO.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HABP4 antibody 33317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |