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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAAO Polyclonal antibody

HAAO Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12791-1-AP

Synonyms

3 HAO, HAAO, HAD, HAO

Validation Data Gallery

Applications:
  • WB analysis of human liver using 12791-1-AP

    WB analysis of human liver using 12791-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 12791-1-AP

    WB analysis of mouse liver using 12791-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 12791-1-AP

    WB analysis of rat liver using 12791-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12791-1-AP

    IHC staining of human gliomas using 12791-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human gliomas using 12791-1-AP

    IHC staining of human gliomas using 12791-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 12791-1-AP

    IF Staining of HepG2 using 12791-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12791-1-AP (HAAO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12791-1-AP targets HAAO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAAO fusion protein Ag3511 相同性解析による交差性が予測される生物種
Full Name 3-hydroxyanthranilate 3,4-dioxygenase
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC029510
Gene symbol HAAO
Gene ID (NCBI) 23498
RRIDAB_2115432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAAO antibody 12791-1-APDownload protocol
IHC protocol for HAAO antibody 12791-1-APDownload protocol
IF protocol for HAAO antibody 12791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Adv Sci (Weinh)

Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth

Authors - Dong Liu
  • KO Validated
View Article
ratIHC

BMC Genomics

Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats

Authors - Wang Zhu
View Article
humanWB

Nat Commun

Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma

Authors - Miyuki Nomura
View Article