Validation Data Gallery
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Tested Applications
| Positive WB detected in | human liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
Product Information
12791-1-AP targets HAAO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3511 Product name: Recombinant human HAAO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC029510 Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG 相同性解析による交差性が予測される生物種 |
| Full Name | 3-hydroxyanthranilate 3,4-dioxygenase |
| Calculated molecular weight | 286 aa, 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC029510 |
| Gene Symbol | HAAO |
| Gene ID (NCBI) | 23498 |
| RRID | AB_2115432 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HAAO antibody 12791-1-AP | Download protocol |
| IHC protocol for HAAO antibody 12791-1-AP | Download protocol |
| WB protocol for HAAO antibody 12791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Genomics Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats | ||
Gut Microbes Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila | ||
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
Environ Pollut Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis | ||
Nat Commun Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma |