Validation Data Gallery
Tested Applications
| Positive WB detected in | human liver tissue, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
Product Information
12791-1-AP targets HAAO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3511 Product name: Recombinant human HAAO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC029510 Sequence: MERRLGVRAWVKENRGSFQPPVCNKLMHQEQLKVMFIGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGKHRDVVIRQGEIFLLPARVPHSPQRFANTVGLVVERRRLETELDGLRYYVGDTMDVLFEKWFYCKDLGTQLAPIIQEFFSSEQYRTGKPIPDQLLKEPPFPLSTRSIMEPMSLDAWLDSHHRELQAGTPLSLFGDTYETQVIAYGQGSSEGLRQNVDVWLWQLEGSSVVTMGGRRLSLAPDDSLLVLAGTSYAWERTQGSVALSVTQDPACKKPLG 相同性解析による交差性が予測される生物種 |
| Full Name | 3-hydroxyanthranilate 3,4-dioxygenase |
| Calculated molecular weight | 286 aa, 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC029510 |
| Gene Symbol | HAAO |
| Gene ID (NCBI) | 23498 |
| RRID | AB_2115432 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HAAO is also known by several synonyms, including 3-HAO (3-hydroxyanthranilate oxygenase), HAO (3-hydroxyanthranilic acid dioxygenase), h3HAO and HAD. HAAO is widely distributed in peripheral organs, with particularly high expression in the liver and kidney. Strong positivity has been observed in the cytoplasm of hepatocytes and renal tubules, as well as in cilia of respiratory epithelium and fallopian tube epithelium. Moderate staining is detected in the small intestine and subsets of lymphoid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HAAO antibody 12791-1-AP | Download protocol |
| IHC protocol for HAAO antibody 12791-1-AP | Download protocol |
| WB protocol for HAAO antibody 12791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gut Microbes Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila | ||
Environ Pollut Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis | ||
Nat Commun Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma | ||
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
BMC Genomics Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats |