Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82819-2-RR targets H6PD in WB, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | H6PD fusion protein Ag7109 相同性解析による交差性が予測される生物種 |
| Full Name | hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) |
| Calculated molecular weight | 89 kDa |
| Observed molecular weight | 89-95 kDa |
| GenBank accession number | BC081559 |
| Gene Symbol | H6PD |
| Gene ID (NCBI) | 9563 |
| RRID | AB_3670562 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95479 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for H6PD antibody 82819-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |