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KD/KO Validated

Histone H2A.z Polyclonal antibody

Name: Histone H2A.z Polyclonal antibody
Brand: Proteintech
SKU: 16441-1-AP

Histone H2A.z Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16441-1-AP

Synonyms

H2A histone family, member Z, H2A.z, H2A/z, H2AFZ, H2AZ, histone, Histone H2A, Histone H2A.Z

Validation Data Gallery

Applications:

AllWBIF

WB analysis of human brain using 16441-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16441-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16441-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 16441-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 16441-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue, mouse kidney tissue
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 3 publications below

Product Information

16441-1-AP targets Histone H2A.z in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, Oratosquilla oratoria, mouse, fish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Histone H2A.z fusion protein Ag9762 相同性解析による交差性が予測される生物種
Full Name	H2A histone family, member Z
Calculated molecular weight	128 aa, 14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC018002
Gene symbol	H2AFZ
Gene ID (NCBI)	3015
RRID	AB_2115113
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H2A.z antibody 16441-1-AP	Download protocol
IF protocol for Histone H2A.z antibody 16441-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
fish	IF	Sci Adv The lungfish cocoon is a living tissue with antimicrobial functions. Authors - Ryan Darby Heimroth View Article
mouse	WB	J Biol Chem The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes. Authors - Haixin Zhao KD Validated View Article
human	WB	Biomed Pharmacother Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness. Authors - Ling Gao View Article
mouse	IF,WB	Biol Open Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway. Authors - BreAnna Cameron View Article
Oratosquilla oratoria	IF	J Morphol Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda). Authors - Tingrong Chen View Article
human	WB	Front Oncol DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway Authors - Zhitao Han View Article

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企業情報

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H2A.z Polyclonal antibody

Histone H2A.z Polyclonal Antibody for WB, IF, ELISA

Validation Data Gallery

WB analysis of human brain using 16441-1-AP

WB analysis of mouse kidney using 16441-1-AP

WB analysis of HepG2 using 16441-1-AP

IF Staining of HeLa using 16441-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The lungfish cocoon is a living tissue with antimicrobial functions.

The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.

Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.

Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway.

Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).

DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway