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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

H2AFY Polyclonal antibody

H2AFY Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 26875-1-AP

Synonyms

Core histone macro H2A.1, H2A histone family, member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2AFY, Histone H2A.y, Histone macroH2A1, MACROH2A1, MACROH2A1.1, macroH2A1.2, mH2A1

Validation Data Gallery

Applications:
  • WB analysis using 26875-1-AP

    WB analysis using 26875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 26875-1-AP

    WB analysis of A549 using 26875-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Caco-2 using 26875-1-AP

    WB analysis of Caco-2 using 26875-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 26875-1-AP

    WB analysis of A549 using 26875-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 26875-1-AP

    WB analysis of mouse brain using 26875-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26875-1-AP

    IF Staining of HepG2 using 26875-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, mouse brain tissue, HeLa cells, rat brain
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26875-1-AP is H2AFY Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen H2AFY fusion protein Ag25445 相同性解析による交差性が予測される生物種
Full Name H2A histone family, member Y
Calculated molecular weight 372 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC013331
Gene symbol H2AFY
Gene ID (NCBI) 9555
RRIDAB_2918113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for H2AFY antibody 26875-1-APDownload protocol
IF protocol for H2AFY antibody 26875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Cell Death Discov

CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation

Authors - Ting Zhao
View Article