Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19321-1-AP targets H1FX in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5819 Product name: Recombinant human H1FX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC000426 Sequence: MSVELEEALPVTTAEGMAKKVTKAGGSAALSPSKKRKNSKKKNQPGKYSQLVVETIRRLGERNGSSLAKIYTEAKKVPWFDQQNGRTYLKYSIKALVQNDTLLQVKGTGANGSFKLNRKKLEGGGERRGAPAAATAPAPTAHKAKKAAPGAAGSRRADKKPARGQKPEQRSHKKGAGAKKDKGGKAKKTAAAGGKKVKKAAKPSVPKVPK 相同性解析による交差性が予測される生物種 |
| Full Name | H1 histone family, member X |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC000426 |
| Gene Symbol | H1FX |
| Gene ID (NCBI) | 8971 |
| RRID | AB_3741959 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92522 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
H1FX (Histone H1x) belongs to the histone H1/H5 family which plays a crucial role in the organization and regulation of chromatin structure.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for H1FX antibody 19321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |