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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using Granzyme B Polyclonal antibody (13588-1-AP)

    WB analysis of HL-60 using 13588-1-AP (same clone as 13588-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13588-1-AP (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody (13588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Granzyme B Polyclonal antibody (13588-1-AP)

    IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody (13588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Granzyme B Polyclonal antibody (13588-1-AP)

    IF Staining of Jurkat using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Granzyme B antibody (13588-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Granzyme B Polyclonal antibody (13588-1-AP)

    IF Staining of MCF-7 using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Granzyme B antibody (13588-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

Granzyme B Polyclonal antibody, PBS Only

Granzyme B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 13588-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13588-1-PBS targets Granzyme B in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3883

Product name: Recombinant human GZMB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-247 aa of BC030195

Sequence: MQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIQDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRH

相同性解析による交差性が予測される生物種
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC030195
Gene Symbol Granzyme B
Gene ID (NCBI) 3002
RRIDAB_2114429
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10144
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

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