Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human brain tissue, mouse brain tissue, rat brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 4 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
Product Information
26950-1-AP targets GNRH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25667 Product name: Recombinant human GNRH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-92 aa of BC126463 Sequence: QHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQPRSPLRDLKGALESLIEEETGQKKI 相同性解析による交差性が予測される生物種 |
| Full Name | gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) |
| Calculated molecular weight | 92 aa, 10 kDa |
| GenBank accession number | BC126463 |
| Gene Symbol | GNRH1 |
| Gene ID (NCBI) | 2796 |
| RRID | AB_2880698 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GNRH1 antibody 26950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Microglia Rank signaling regulates GnRH neuronal function and the hypothalamic-pituitary-gonadal axis. | ||
Cell Metab Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice | ||
Nat Neurosci A GnRH neuronal population in the olfactory bulb translates socially relevant odors into reproductive behavior in male mice | ||
NPJ Biofilms Microbiomes Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice | ||
EBioMedicine Establishing the role of ZBTB20 mutations in GnRH deficiency and impaired neurogenesis in the subventricular zone: a human cohort and animal model study. | ||
JCI Insight Investigation of a mouse model of Prader-Willi syndrome with combined disruption of Necdin and Magel2 |