Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, rat kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86604-3-RR targets Glycerokinase in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4175 Product name: Recombinant human GK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC037549 Sequence: MAASKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEKTCEKLGQLNIDISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKQLCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAKEVGTSYG 相同性解析による交差性が予測される生物種 |
| Full Name | glycerol kinase |
| Calculated molecular weight | 524 aa, 57 kDa |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | BC037549 |
| Gene Symbol | Glycerokinase |
| Gene ID (NCBI) | 2710 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P32189 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycerokinase, also known as glycerol kinase (GK, EC 2.7.1.30), is a widely conserved phosphotransferase that catalyzes the ATP-dependent phosphorylation of glycerol to generate sn-glycerol-3-phosphate (G3P). Glycerokinase exists as four isoforms with apparent molecular masses of 61 kDa, 57 kDa, 61 kDa, and 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Glycerokinase antibody 86604-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |