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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    WB analysis of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

    WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    WB analysis using 11037-2-AP (same clone as 11037-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    WB analysis using 11037-2-AP (same clone as 11037-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IP experiment of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

    IP result of anti-Glutamine Synthetase (IP:11037-2-AP, 4ug; Detection:11037-2-AP 1:5000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IF Staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IF Staining of mouse eye using 11037-2-AP (same clone as 11037-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IF Staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human brain tissue using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    IF Staining of human brain using 11037-2-AP (same clone as 11037-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    FC experiment of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Glutamine Synthetase Polyclonal antibody (11037-2-AP)

    FC experiment of HepG2 using 11037-2-AP (same clone as 11037-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Polyclonal antibody, PBS Only

Glutamine Synthetase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA
Cat No. 11037-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11037-2-PBS targets Glutamine Synthetase in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1510

Product name: Recombinant human Glutamine synthetase protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC011700

Sequence: MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2110650
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP15104
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

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