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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Glutamate receptor 2 Polyclonal antibody (11994-1-AP)

    WB analysis using 11994-1-AP (same clone as 11994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Glutamate receptor 2 Polyclonal antibody (11994-1-AP)

    IP experiment of mouse brain using 11994-1-AP (same clone as 11994-1-PBS)

    IP result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:2000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Glutamate receptor 2 Polyclonal antibody (11994-1-AP)

    IHC staining of mouse brain using 11994-1-AP (same clone as 11994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Glutamate receptor 2 Polyclonal antibody (11994-1-AP)

    IF Staining of mouse eye using 11994-1-AP (same clone as 11994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Glutamate receptor 2 Polyclonal antibody (11994-1-AP)

    IF Staining of mouse eye using 11994-1-AP (same clone as 11994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

Glutamate receptor 2 Polyclonal antibody, PBS Only

Glutamate receptor 2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 11994-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

GRIA2, AMPA-selective glutamate receptor 2, GluA2, GluR 2, GluR B

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11994-1-PBS targets Glutamate receptor 2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2662

Product name: Recombinant human GRIA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-356 aa of BC010574

Sequence: GADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLVEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTTTIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGL

相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, AMPA 2
Calculated molecular weight 883 aa, 99 kDa
Observed molecular weight 94-105 kDa
GenBank accession numberBC010574
Gene Symbol GRIA2
Gene ID (NCBI) 2891
RRIDAB_2113725
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP42262
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.

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