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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glucocorticoid receptor Recombinant antibody, PBS Only

Glucocorticoid receptor Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F22

Cat no : 82619-4-PBS

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

Applications:
  • WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

82619-4-PBS targets Glucocorticoid receptor in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 94-97 kDa
GenBank accession numberBC015610
Gene symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).