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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    WB analysis of mouse skin using 16730-1-AP (same clone as 16730-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    WB analysis of mouse small intestine using 16730-1-AP (same clone as 16730-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of human cervix using 16730-1-AP (same clone as 16730-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of human cervix using 16730-1-AP (same clone as 16730-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of mouse skin using 16730-1-AP (same clone as 16730-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16730-1-AP (Galectin-7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of mouse skin using 16730-1-AP (same clone as 16730-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16730-1-AP (Galectin-7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16730-1-PBS in a different storage buffer formulation.

Galectin-7 Polyclonal antibody, PBS Only

Galectin-7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16730-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

LGALS7B, Gal 7, GAL7, Gal-7, Galectin 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16730-1-PBS targets Galectin-7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10188

Product name: Recombinant human Galectin-7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-136 aa of BC073743

Sequence: MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 7B
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 14-15 kDa
GenBank accession numberBC073743
Gene Symbol Galectin-7
Gene ID (NCBI) 653499
RRIDAB_10596781
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP47929
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-7 contains one carbohydrate recognition domain (CRD) and is biologically active as monomer. It is mainly expressed in stratified epithelium, especially in epidermis. Alterations of galectin-7 expression during cancer progression have been reported. Galectin-7 is involved in epithelial differentiation and development and may have a role in tumour development (PMID: 16429325).

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