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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Galectin-3 Polyclonal antibody (14979-1-AP)

    WB analysis of HeLa cells using 14979-1-AP (same clone as 14979-1-PBS)

    WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Galectin-3 Polyclonal antibody (14979-1-AP)

    WB analysis using 14979-1-AP (same clone as 14979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Galectin-3 Polyclonal antibody (14979-1-AP)

    WB analysis of human heart using 14979-1-AP (same clone as 14979-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using Galectin-3 Polyclonal antibody (14979-1-AP)

    WB analysis of rat colon using 14979-1-AP (same clone as 14979-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using Galectin-3 Polyclonal antibody (14979-1-AP)

    IP experiment of MCF-7 using 14979-1-AP (same clone as 14979-1-PBS)

    IP result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Polyclonal antibody (14979-1-AP)

    IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Polyclonal antibody (14979-1-AP)

    IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-3 Polyclonal antibody (14979-1-AP)

    IHC staining of human colon using 14979-1-AP (same clone as 14979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Galectin-3 Polyclonal antibody (14979-1-AP)

    IF Staining of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin-3 antibody (14979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Galectin-3 Polyclonal antibody (14979-1-AP)

    FC experiment of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Galectin-3 Polyclonal antibody (14979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14979-1-PBS targets Galectin-3 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-3 fusion protein Ag6891 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene Symbol Galectin-3
Gene ID (NCBI) 3958
RRIDAB_2136768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17931
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).