Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse small intestine tissue |
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87897-1-RR targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg7632 Product name: Recombinant human LGALS2 protein Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 2-132 aa of BC059782 Sequence: TGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE 相同性解析による交差性が予測される生物種 |
| Full Name | lectin, galactoside-binding, soluble, 2 |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 10-15 kDa |
| GenBank accession number | BC059782 |
| Gene Symbol | Galectin 2 |
| Gene ID (NCBI) | 3957 |
| RRID | AB_3745814 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a class of carbohydrate-binding proteins implicated in multiple physiological processes including inflammation, immune responses, cell migration, autophagy and signal transduction (PMID: 29717004). Galectin-2 (Gal-2, also known as LGALS2) is a monocyte-derived carbohydrate-binding lectin; its upregulation correlates with reduced collateral arteriogenesis in patients with obstructive coronary artery disease (PMID: 25884209). Additionally, Gal-2 may interact with T cells, particularly in the setting of inflammatory bowel disease (PMID: 15356130).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Galectin 2 antibody 87897-1-RR | Download protocol |
| WB protocol for Galectin 2 antibody 87897-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |