Validation Data Gallery
Tested Applications
| Positive WB detected in | human blood |
| Positive IHC detected in | human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18147-1-AP targets GYPC in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GYPC fusion protein Ag12825 相同性解析による交差性が予測される生物種 |
| Full Name | glycophorin C (Gerbich blood group) |
| Calculated molecular weight | 128 aa, 14 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC106051 |
| Gene Symbol | GYPC |
| Gene ID (NCBI) | 2995 |
| RRID | AB_2878508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GYPC antibody 18147-1-AP | Download protocol |
| IHC protocol for GYPC antibody 18147-1-AP | Download protocol |
| IF protocol for GYPC antibody 18147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology |