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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A/CD235a Recombinant antibody (85182-3-RR)

    IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A/CD235a Recombinant antibody (85182-3-RR)

    IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01888-1

    Cytometric bead array standard curve of MP01888-1

    Cytometric bead array standard curve of MP01888-1, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-2-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01888-2

    Cytometric bead array standard curve of MP01888-2

    Cytometric bead array standard curve of MP01888-2, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-1-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

Glycophorin A/CD235a Recombinant monoclonal antibody, PBS Only (Detector)

Glycophorin A/CD235a Uni-rAb® Recombinant Antibody for IHC, Cytometric bead array, Indirect ELISA
Cat No. 85182-3-PBS
Clone No.242822H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Cytometric bead array, Indirect ELISA

Synonyms

Glycophorin A, 242822H5, CD235a, Glycophorin-A, GPA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85182-3-PBS targets Glycophorin A/CD235a as part of a matched antibody pair:

MP01888-1: 85182-2-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

MP01888-2: 85182-1-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2336

Product name: Recombinant Human Glycophorin A protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-91 aa of BC005319

Sequence: SSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPE

相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
GenBank accession numberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02724
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). Glycophorin A represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)