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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HEK-293 using 67047-1-Ig

    WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of Jurkat using 67047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of fetal human brain using 67047-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of rat brain using 67047-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of mouse brain using 67047-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HeLa using 67047-1-Ig

    WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HEK-293 using 67047-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of Jurkat using 67047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human thyroid cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human prostate cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human thyroid cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

GUK1 Monoclonal antibody

GUK1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67047-1-Ig
Clone No.2H5E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

EC:2.7.4.8, GMK, GMP kinase, GMPK, Guanylate kinase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, HeLa cells
Positive IHC detected inhuman thyroid cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman thyroid cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67047-1-Ig targets GUK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28805

Product name: Recombinant human GUK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-197 aa of BC009914

Sequence: MSGPRPVVLSGPSGAGKSTLLKRLLQEHSGIFGFSVSHTTRNPRPGEENGKDYYFVTREVMQRDIAAGDFIEHAEFSGNLYGTSKVAVQAVQAMNRICVLDVDLQGVRNIKATDLRPIYISVQPPSLHVLEQRLRQRNTETEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA

相同性解析による交差性が予測される生物種
Full Name guanylate kinase 1
Calculated molecular weight 22 kDa
Observed molecular weight 22-27 kDa
GenBank accession numberBC009914
Gene Symbol GUK1
Gene ID (NCBI) 2987
RRIDAB_2882360
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ16774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for GUK1 antibody 67047-1-IgDownload protocol
IHC protocol for GUK1 antibody 67047-1-IgDownload protocol
WB protocol for GUK1 antibody 67047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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