Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)
WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
WB analysis using 17489-1-AP (same clone as 17489-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17489-1-AP (same clone as 17489-1-PBS)
Hela cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 17489-1-AP (same clone as 17489-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 17489-1-AP (same clone as 17489-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 17489-1-AP (same clone as 17489-1-PBS)
IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)
Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GUF1 Polyclonal antibody, PBS Only

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17489-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17489-1-PBS targets GUF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11579 Product name: Recombinant human GUF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-669 aa of BC036768 Sequence: VGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK 相同性解析による交差性が予測される生物種
Full Name	GUF1 GTPase homolog (S. cerevisiae)
Calculated molecular weight	669 aa, 74 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC036768
Gene Symbol	GUF1
Gene ID (NCBI)	60558
RRID	AB_2115960
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N442
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

WB analysis using 17489-1-AP (same clone as 17489-1-PBS)

WB analysis of HeLa using 17489-1-AP (same clone as 17489-1-PBS)

WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

WB analysis of mouse pancreas using 17489-1-AP (same clone as 17489-1-PBS)

WB analysis of mouse brain using 17489-1-AP (same clone as 17489-1-PBS)

IP experiment of HeLa using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human lung cancer using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human kidney using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human kidney using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human testis using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human testis using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

IF Staining of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

GUF1 Polyclonal antibody, PBS Only

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™