Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
55127-1-AP targets GUCY2D in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | guanylate cyclase 2D, membrane (retina-specific) |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 120 kDa, 95 kDa |
| GenBank accession number | NM_000180 |
| Gene Symbol | GUCY2D |
| Gene ID (NCBI) | 3000 |
| RRID | AB_10804281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02846 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
GUCY2D, also named as CORD6, GUC1A4, GUC2D, RETGC, RETGC1 and ROS-GC, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. A number of nonsense and frameshift mutations in the GUCY2D gene have been identified in LCA1 patients. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). GUCY2D is responsible for many reported cases of autosomal dominant CRDs. The antibody is specific to GUCY2D.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GUCY2D antibody 55127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
J Biol Chem The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. |