Validation Data Gallery
Tested Applications
| Positive IP detected in | A549 cells |
| Positive IF/ICC detected in | U2OS cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19813-1-AP targets GTPBP8 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13848 Product name: Recombinant human GTPBP8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC000003 Sequence: WEGRMAAPGLRLGAGRLFEMPAVLERLSRYNSTSQAFAEVLRLPKQQLRKLLYPLQEVERFLAPYGRQDLHLRIFDPSPEDIARADNIFTATERNRIDYVSSAVRIDHAPDLPRPEVCFIGRSNVGKSSLIKALFSLAPEVEVRVSKKPGHTKKMNFFKVGKHFTAGGHAR 相同性解析による交差性が予測される生物種 |
| Full Name | GTP-binding protein 8 (putative) |
| Calculated molecular weight | 284 aa, 32 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC000003 |
| Gene Symbol | GTPBP8 |
| Gene ID (NCBI) | 29083 |
| RRID | AB_3741969 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3Z3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTPBP8 is a protein-coding gene encoding a GTP-binding protein that plays a critical role in mitochondrial ribosome (mitoribosome) biogenesis . It is the human homolog of the bacterial EngB family of GTPases, which are involved in ribosomal large subunit assembly. (PMID: 38969660)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GTPBP8 antibody 19813-1-AP | Download protocol |
| IP protocol for GTPBP8 antibody 19813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |