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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using GTPBP2 Polyclonal antibody (11557-1-AP)

    WB analysis of human kidney using 11557-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using GTPBP2 Polyclonal antibody (11557-1-AP)

    WB analysis of human spleen using 11557-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using GTPBP2 Polyclonal antibody (11557-1-AP)

    IP experiment of mouse spleen using 11557-1-AP

    IP result of anti-GTPBP2 (IP:11557-1-AP, 4ug; Detection:11557-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GTPBP2 Polyclonal antibody (11557-1-AP)

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using GTPBP2 Polyclonal antibody (11557-1-AP)

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 40x lens).

GTPBP2 Polyclonal antibody

GTPBP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11557-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

GTP binding protein 2, GTPBP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue, human spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11557-1-AP targets GTPBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2113

Product name: Recombinant human GTPBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-532 aa of BC028347

Sequence: SKMITFIDLAGHHKYLHTTIFGLTSYCPDCALLLVSANTGIAGTTREHLGLALALKVPFFIVVSKIDLCAKTTVERTVRQLERVLKQPGCHKVPMLVTSEDDAVTAAQQFAQSPNVTPIFTLSSVSGESLDLLKVFLNILPPLTNSKEQEELMQQLTEFQVDEIYTVPEVGTVVGGTLSSGICREGDQLVVGPTDDGCFLELRVCSIQRNRSACRVLRAGQAATLALGDFDRALLRKGMVMLSPEMNPTICSVFEAEIVLLFHATTFRRGFQVTVHVGNVRQTAVVEKIHAKDKLRTGEKAVVRFRFLKHPEYLKVGAKLLFREGVTKGIGHVTDVQAITAGEAQANMGF

相同性解析による交差性が予測される生物種
Full Name GTP binding protein 2
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC028347
Gene Symbol GTPBP2
Gene ID (NCBI) 54676
RRIDAB_2115381
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX10
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTPBP2 antibody 11557-1-APDownload protocol
IHC protocol for GTPBP2 antibody 11557-1-APDownload protocol
IP protocol for GTPBP2 antibody 11557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols