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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GTPBP1 Polyclonal antibody

GTPBP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16374-1-AP

Synonyms

G protein 1, GP 1, GP1, GTP binding protein 1, GTPBP1, HSPC018

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 16374-1-AP

    WB analysis of mouse skeletal muscle using 16374-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16374-1-AP (GTPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 16374-1-AP

    WB analysis of Jurkat using 16374-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16374-1-AP (GTPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 16374-1-AP

    IP experiment of mouse skeletal muscle using 16374-1-AP

    IP result of anti-GTPBP1 (IP:16374-1-AP, 4ug; Detection:16374-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, Jurkat cells
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

16374-1-AP targets GTPBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTPBP1 fusion protein Ag9332 相同性解析による交差性が予測される生物種
Full Name GTP binding protein 1
Calculated molecular weight 660 aa, 71 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC014075
Gene symbol GTPBP1
Gene ID (NCBI) 9567
RRIDAB_2115267
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTPBP1 antibody 16374-1-APDownload protocol
IP protocol for GTPBP1 antibody 16374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols