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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GTF3C6 Polyclonal antibody (26382-1-AP)

    WB analysis of HeLa using 26382-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GTF3C6 Polyclonal antibody (26382-1-AP)

    WB analysis of HepG2 using 26382-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GTF3C6 Polyclonal antibody (26382-1-AP)

    WB analysis of K-562 using 26382-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GTF3C6 Polyclonal antibody (26382-1-AP)

    IP experiment of HepG2 using 26382-1-AP

    IP result of anti-GTF3C6 (IP:26382-1-AP, 4ug; Detection:26382-1-AP 1:2000) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using GTF3C6 Polyclonal antibody (26382-1-AP)

    IHC staining of human urothelial carcinoma using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GTF3C6 Polyclonal antibody (26382-1-AP)

    IHC staining of mouse lung using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GTF3C6 Polyclonal antibody (26382-1-AP)

    IHC staining of human lung cancer using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GTF3C6 Polyclonal antibody

GTF3C6 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26382-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

TFIIIC35, NPD020, CDA020, C6orf51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human lung cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26382-1-AP targets GTF3C6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23895

Product name: Recombinant human GTF3C6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-213 aa of BC011593

Sequence: MAAAADERSPEDGEDEEEEEQLVLVELSGIIDSDFLSKCENKCKVLGIDTERPILQVDSCVFAGEYEDTLGTCVIFEENVEHADTEGNNKTVLKYKCHTMKKLSMTRTLLTEKKEGEENIGGVEWLQIKDNDFSYRPNMICNFLHENEDEEVVASAPDKSLELEEEEIQMNDSSNLSCEQEKPMHLEIEDSGPLIDIPSETEGSVFMETQMLP

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIIC, polypeptide 6, alpha 35kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC011593
Gene Symbol GTF3C6
Gene ID (NCBI) 112495
RRIDAB_2880497
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF3C6 antibody 26382-1-APDownload protocol
IHC protocol for GTF3C6 antibody 26382-1-APDownload protocol
IP protocol for GTF3C6 antibody 26382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EPMA J

Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies

Authors - Jinjin Zhang
View Article