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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GTF3C4 Polyclonal antibody (17653-1-AP)

    IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GTF3C4 Polyclonal antibody (17653-1-AP)

    IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

GTF3C4 Polyclonal antibody, PBS Only

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17653-1-PBS targets GTF3C4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11898

Product name: Recombinant human GTF3C4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 473-822 aa of BC094774

Sequence: GSVRTHGIAVSPCGAYLAIITTEGMINGLHPVNKNYQVQFVTLKTFEEAAAQLLESSVQNLFKQVDLIDLVRWKILKDKHIPQFLQEALEKKIESSGVTYFWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPTTGDAGGREPMEEKLLEIQGKIEAVEMHLTREHMKRVLGEVYLHTWITENTSIPTRGLCNFLMSDEEYDDRTARVLIGHISKKMNKQTFPEHCSLCKEILPFTDRKQAVCSNGHIWLRCFLTYQSCQSLIYRRCLLHDSIARHPAPEDPDWIKRLLQSPCPFCDSPVF

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIIC, polypeptide 4, 90kDa
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC094774
Gene Symbol GTF3C4
Gene ID (NCBI) 9329
RRIDAB_2115253
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKN8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.

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