• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GTF3C2 Polyclonal antibody (27494-1-AP)

    WB analysis using 27494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GTF3C2 Polyclonal antibody (27494-1-AP)

    WB analysis of A549 using 27494-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human thyroid cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human cervical cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human cervical cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human cervical cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human cervical cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human thyroid cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human thyroid cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GTF3C2 Polyclonal antibody (27494-1-AP)

    IHC staining of human thyroid cancer using 27494-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GTF3C2 Polyclonal antibody (27494-1-AP)

    IF Staining of HeLa using 27494-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GTF3C2 antibody (27494-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GTF3C2 Polyclonal antibody

GTF3C2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27494-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TFIIIC110, TFIIIC BETA, TF3C-beta, TF3C beta, KIAA0011

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman thyroid cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27494-1-AP targets GTF3C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF3C2 fusion protein Ag26666 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIIC, polypeptide 2, beta 110kDa
Calculated molecular weight 101 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC020981
Gene Symbol GTF3C2
Gene ID (NCBI) 2976
RRIDAB_2880889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

Protocols

Product Specific Protocols
WB protocol for GTF3C2 antibody 27494-1-APDownload protocol
IHC protocol for GTF3C2 antibody 27494-1-APDownload protocol
IF protocol for GTF3C2 antibody 27494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Genet

Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder

Authors - Aiko Iwata-Otsubo
View Article