Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33192-1-AP targets GTF3A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35931 Product name: Recombinant human GTF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-365 aa of NM_002097 Sequence: KTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG 相同性解析による交差性が予測される生物種 |
| Full Name | general transcription factor IIIA |
| Calculated molecular weight | 41kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | NM_002097 |
| Gene Symbol | GTF3A |
| Gene ID (NCBI) | 2971 |
| RRID | AB_3742887 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q92664 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTF3A encodes general transcription factor IIIA (TFIIIA) whose role in transcriptional regulation of 5S ribosomal DNA genes (encoding 5S ribosomal RNA (rRNA)) has been well characterized. TFIIIA comprises nine zinc finger (ZF) domains, each containing two invariant pairs of cysteines and histidines coordinating one Zn2+ ion (PMID: 36399538).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF3A antibody 33192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |