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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTF2H3 Polyclonal antibody (14857-1-AP)

    WB analysis using 14857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14857-1-AP (GTF2H3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GTF2H3 Polyclonal antibody

GTF2H3 Polyclonal Antibody for WB, ELISA
Cat No. 14857-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BTF2, BTF2 p34, GTF2H3, TFB4, TFIIH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14857-1-AP targets GTF2H3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2H3 fusion protein Ag6652 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIH, polypeptide 3, 34kDa
Calculated molecular weight 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC065250
Gene Symbol GTF2H3
Gene ID (NCBI) 2967
RRIDAB_3669194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13889
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2H3 antibody 14857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols