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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GTF2H2C Polyclonal antibody

GTF2H2C Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17959-1-AP

Synonyms

GTF2H2C, GTF2H2D

Validation Data Gallery

Applications:
  • WB analysis using 17959-1-AP

    WB analysis using 17959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 17959-1-AP

    WB analysis of HepG2 using 17959-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17959-1-AP

    WB analysis of HeLa using 17959-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 17959-1-AP

    WB analysis of MCF-7 using 17959-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 17959-1-AP

    WB analysis of K-562 using 17959-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 17959-1-AP

    IP experiment of HepG2 using 17959-1-AP

    IP result of anti-GTF2H2C (IP:17959-1-AP, 4ug; Detection:17959-1-AP 1:500) with HepG2 cells lysate 2240 ug.

Tested Applications

Positive WB detected inL02 cells, HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

17959-1-AP targets GTF2H2C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2H2C fusion protein Ag12498 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIH, polypeptide 2C
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC105980
Gene symbol GTF2H2C
Gene ID (NCBI) 728340
RRIDAB_2114793
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2H2C antibody 17959-1-APDownload protocol
IP protocol for GTF2H2C antibody 17959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols