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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTF2H2C Polyclonal antibody (17959-1-AP)

    WB analysis using 17959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GTF2H2C Polyclonal antibody (17959-1-AP)

    WB analysis of HepG2 using 17959-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GTF2H2C Polyclonal antibody (17959-1-AP)

    WB analysis of HeLa using 17959-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GTF2H2C Polyclonal antibody (17959-1-AP)

    WB analysis of MCF-7 using 17959-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GTF2H2C Polyclonal antibody (17959-1-AP)

    WB analysis of K-562 using 17959-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GTF2H2C Polyclonal antibody (17959-1-AP)

    IP experiment of HepG2 using 17959-1-AP

    IP result of anti-GTF2H2C (IP:17959-1-AP, 4ug; Detection:17959-1-AP 1:500) with HepG2 cells lysate 2240 ug.

GTF2H2C Polyclonal antibody

GTF2H2C Polyclonal Antibody for WB, IP, ELISA
Cat No. 17959-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

GTF2H2C, GTF2H2D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17959-1-AP targets GTF2H2C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12498

Product name: Recombinant human GTF2H2C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-395 aa of BC105980

Sequence: MDEEPERTKRWEGGYERTWEILKEDESGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKEAVDMTCHGEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKAAKIRVSVIGLSAEVRVCTVLARETGGTYHVILDESHYKELLTHHLSPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDGNTEPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEIPLEEYNGERFCYGCQGELKDQHVYVCAVCQNVFCVDCDVFVHDSLHCCPGCIHKIPAPSGV

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIH, polypeptide 2C
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC105980
Gene Symbol GTF2H2C
Gene ID (NCBI) 728340
RRIDAB_2114793
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for GTF2H2C antibody 17959-1-APDownload protocol
WB protocol for GTF2H2C antibody 17959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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