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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GTF2E1 Polyclonal antibody (24405-1-AP)

    WB analysis of HEK-293 using 24405-1-AP (same clone as 24405-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24405-1-AP (GTF2E1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of rat kidney using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of rat kidney using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of mouse kidney using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of mouse kidney using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human stomach cancer using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human stomach cancer using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human urothelial carcinoma using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human urothelial carcinoma using 24405-1-AP (same clone as 24405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24405-1-PBS in a different storage buffer formulation.

GTF2E1 Polyclonal antibody, PBS Only

GTF2E1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24405-1-PBS targets GTF2E1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19794

Product name: Recombinant human GTF2E1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 88-439 aa of BC156997

Sequence: RHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE

相同性解析による交差性が予測される生物種
Full Name general transcription factor IIE, polypeptide 1, alpha 56kDa
Calculated molecular weight 439 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC156997
Gene Symbol GTF2E1
Gene ID (NCBI) 2960
RRIDAB_2879526
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29083
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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